منابع مشابه
Annexins, and GTP Modulate Exocytosis from Maize Root Cap Protoplasts
Protoplasts isolated from root cap cells of maize were shown to secrete fucose-rich polysaccharides and were used in a patch–clamp study to monitor changes in whole-cell capacitance. Ca 2 1 was required for exocytosis, which was measured as an increase in cell capacitance during intracellular dialysis with Ca 2 1 buffers via the patch pipette. Exocytosis was stimulated significantly by small in...
متن کاملSignal transduction in maize and Arabidopsis mesophyll protoplasts.
Plant protoplasts show physiological perceptions and responses to hormones, metabolites, environmental cues, and pathogen-derived elicitors, similar to cell-autonomous responses in intact tissues and plants. The development of defined protoplast transient expression systems for high-throughput screening and systematic characterization of gene functions has greatly contributed to elucidating pla...
متن کاملHeat Inducible Expression of a Chimeric Maize hsp70CAT Gene in Maize Protoplasts.
The response of maize (Zea mays L.) protoplasts to high temperature stress was investigated. After isolation and electroporation, protoplasts were preincubated for 12 hours at 26 degrees C then incubated for 6 hours at elevated temperatures. The pattern of polypeptides synthesized by these protoplasts during the last hour was monitored by in vivo labeling with (35)S-methionine. Incubation at 40...
متن کاملCa2+, annexins, and GTP modulate exocytosis from maize root cap protoplasts
Protoplasts isolated from root cap cells of maize were shown to secrete fucose-rich polysaccharides and were used in a patch-clamp study to monitor changes in whole-cell capacitance. Ca2+ was required for exocytosis, which was measured as an increase in cell capacitance during intracellular dialysis with Ca2+ buffers via the patch pipette. Exocytosis was stimulated significantly by small increa...
متن کاملSeparation of mesophyll protoplasts and bundle sheath cells from maize leaves for photosynthetic studies.
Mesophyll protoplasts and bundle sheath strands of maize (Zea mays L.) leaves have been isolated by enzymatic digestion with cellulase. Mesophyll protoplasts, enzymatically released from maize leaf segments, were further purified by use of a polyethylene glycol-dextran liquid-liquid two phase system. Bundle sheath strands released from the leaf segments were isolated using filtration techniques...
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ژورنال
عنوان ژورنال: Planta
سال: 1987
ISSN: 0032-0935,1432-2048
DOI: 10.1007/bf00395067